Three new monoterpenoid indole alkaloids, 18, 19-dihydro-21-oxokoumine (1), 14-hydroxygelselegine (2) and N-b-methyl-19, 20-dihydrorankinidine (3), together with five known alkaloids (4-8) were isolated from the roots of Gelsemium elegans. The structures with absolute configurations of the new compounds were elucidated on the basis of HR-ESI-MS, NMR, CD spectra analysis and single crystal X-ray diffraction.