A novel croomine derivative, tuberospironine A (3-epi-tuberospironine) was isolated from the root extracts of Stemona tuberosa Lour. found growing on Seram Island, Moluccas Province, Indonesia. The structure of this novel alkaloid, with unprecedented configuration at C-3 for a croomine derivative, was determined from interpretation of its NMR spectroscopic data. © 2012 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.