From the aerial parts of Nauclea officinalis (Pierre ex Pitard) Merr. et Chun, one new indole alkaloid paratunamide E (1) has been isolated together with six known indole alkaloids, paratunamide A (2), nauclealotide C (3), nauclealotide A (4), vincosamide (5), strictosamide (6) and naucleamide F (7). Naucleamide F (7) was isolated from Nauclea officinalis for the first time. All of the seven compounds above were elucidated by spectroscopic methods including 1 D and 2 D NMR spectroscopic analyses. © 2014 Journal of Chinese Pharmaceutical Sciences, School of Pharmaceutical Sciences, Peking University.