Abstract Chemical examination of the fungus Aspergillus aegyptiacus isolated from cotton textile yielded two new pyrrolidine alkaloids: aegyptolidine A (3) and aegyptolidine B (4), together with two known ophiobolin sesterterpenoids: (±)-5-deoxovariecolin (1) and variculanol (2). Compound 1 is reported for the first time from a natural source. The structures of the isolated compounds were unambiguously elucidated on the basis of extensive spectroscopic data analysis (1D (1H and 13C) and 2D (1H-1H COSY, HMQC, HMBC, and NOESY) NMR, MS, IR, and UV) and comparison with literature data. Compounds 1 and 2 showed moderate cytotoxic activity against murine lymphoma cancer cell line L5178Y with ED50 7.6 and 8.1 μg/mL, respectively. © 2015 Phytochemical Society of Europe.