The first phytochemical investigation of the root of Dasymaschalon glaucum led to the isolation of a new oxoaporphine alkaloid 1 together with seven known compounds, duguevalline (2), stigmalactam (3), enterocarpam III (4), desmosdumotin B (5), spiraeamide (6), kaempferol-3-O-glucopyranoside (7) and β-sitosterol (8). The chemical structures of all isolated compounds were established on the basis of their spectroscopic data. The cytotoxic and anti-HIV 1-RT activities of isolated compounds were also evaluated. © 2018 Natural Product Incorporation.