A 3D look at 2D spectra: Two previously unreported indole alkaloids could be rapidly identified from a library of unfractionated fungal extracts by using a newly developed protocol for the differential analysis of arrays of 2D NMR spectra (see picture). The technique thus represents an effective tool for the non-discriminatory characterization of secondary-metabolite mixtures. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.