Cultivated and wild potato species synthesize a wide variety of steroidal glycoalkaloids (GA) that may affect either human health or biotic stress resistance Therefore. GA composition must be a major criterion in the evaluation of breeding products when species genomes are merged and/or manipulated This milk reports the results of GA analysis performed on unique haploid (2n = 2x = 24) plants obtained from tetraploid (2n = 4x = 48) Solarium bulbocastanum - S tuberosum hybrids through in vitro anther culture Glycoalkaloids were extracted from tubers and analyzed by HPLC Haploids generally showed the occurrence of parental GA However, in several cases loss of parental GA and gain of new GA lacking in the parents was observed It may be hypothesized that new GA profiles of our haploids is the result of either genetic recombination or combinatorial biochemistry events To highlight differences between haploids and parents, soluble proteins and antioxidant activities were also determined Both were always higher in haploids compared to then. parents The nature of the newly formed GAs will be further investigated, because they may represent new metabolites that can be used against pest and diseases, or are useful for human health