Five new C-19-diterpenoid alkaloids, named hemsleyaconitines A-E (1-5, resp.), were isolated from Aconitum hemsleyanum PRITZ. By UV, IR, MS, 1D- and 2D-NMR analyses, their structures were elucidated as 18-dehydroxygeniculatine D (1), 6-hydroxy-14-O-veratroylneoline (2), 14-O-acetyl-8-ethoxysachaconitine (3), 18-veratroylkaracoline (4) and 8-O-ethylaustroconitine B (5).