Further investigation on the roots of Aconitum piepunense led to the isolation of a novel bis-diterpenoid alkaloid designated as piepunine (1) Its structure was established by extensive interpretation of its 1D- and 2D NMR data, high-resolution ESI-MS, and IR spectra Piepunine represents the first example of an atisine - denudatine-type bis diterpenoid alkaloids.