Enzyme catalysed oxidation of nazlinin and nazlinin derivatives. Characterisation of the reaction products

Tetrahedron
1996.0

Abstract

Porcine Kidney Diamine Oxidase (PKDO) was used to catalyse the oxidative transformation of Nitraria alkaloids. Nazlinin yielded indoloquinolizidine 4, an alkaloid which has been obtained as a natural product from Nitraria plant extracts. Oxidative deamination of 1-(4-butylamino)-beta-carboline 10 with PKDO yielded an unstable aldehyde (11), which cyclised to a new, arborescidine-type azepine (12). Addition of alcohol dehydrogenase during the oxidative deamination reduced the aldehyde in situ to the corresponding alcohol 13. Topaquinone-analogue 9 was effective as a stoichiometric oxidant for these primary amines. Characterisation of the enzyme reaction products was done by comparison with the chemically synthesised compounds. Copyright (C) 1996 Elsevier Science Ltd

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