One novel bis(monoterpenoid) indole alkaloid glucoside, hirsutaside D (1), along with three known compounds, bahienoside A (2), bahienoside B (3) and neonaucleoside B (4), were isolated from the leaves of U. hirsuta. The structure of compound 1 was elucidated on the basis of extensive spectroscopic data analysis and chemical means. Characterization of compounds 2-4 was based on spectral analysis and comparison with the literature. Their cytotoxic effects on four human tumor cell lines were examined. © 2011 Phytochemical Society of Europe.