Four new alkaloids, pandamarilactonines-E, -F, and -F-N-oxide consisting of a pyrrolidine moiety and two alpha-methyl-gamma-lactone residues, and pandamarilactonine-G, possessing a pyrrolidinone function, were isolated from the roots of Pandanus amaryllifolius. Their structures were determined based on spectroscopic analyses and chemical syntheses. (C) 2010 Elsevier Ltd. All rights reserved.