Further fractionation of C. acuminata has yielded two new alkaloids, hydroxycamptothecin (2) and methoxycamptothecin (3). The former could be methylated to give a methyl ether identical with 3. In order to establish the position of the hydroxyl group in ring A, nmr spectra of deuterated methoxycamptothecin and of the model compounds 7-10 have been obtained. The syntheses of model compounds have been described. © 1969, American Chemical Society. All rights reserved.