Three new pregnane-type steroidal alkaloids salignenamide-A 1 [(20S, 2'Z)-20-(N,N-dimethylamino)-3β-(3',4'-dimethyl-2-pentenamido)-pregnane], salignenamide-B 2 [(20S, 2'Z)-20-(N,N-dimethylamino)-3β-(3'-methyl-2'-butenamido)-pregna-4,14-diene] and 2,4-diacetoxyepipachysamine-D 3 [(20S)20-(N,N-dimethylamino)-3β-benzamido-2β, 4β-diacetoxypregnane] have been isolated from the ethanolic extracts of the whole plant of Sarcococca saligna. The structures of these steroidal alkaloids were established on the basis of detailed spectroscopic studies.