Three minor indole alkaloids have been isolated from ''hairy roots'' of Rauwolfia serpentina Benth.: 3-epi-alpha-yohimbine (1), 18 beta-hydroxy-3-epi-alpha-yohimbine (2), and 12-hydroxyajmaline (3). The latter compound (3) is a novel alkaloid. The alkaloid structures have been determined by spectroscopic data.