Three new steroidal alkaloids were isolated from the bulbs of Fritillaria delavayi franch. the structures of delafrinone (1), delafrine (2) and the third alkaloid were deduced to be (22S,25S)-3β, 16β-dihydroxy-5a-cevanin-6-one, (225,255)-5a-cevanine-3β,6β,16β-triol and (22R,25S)-solanid-5-enine-3β,5a,6α-triol (3), respectively, on the basis of spectral data and chemical evidence. © 1988, The Pharmaceutical Society of Japan. All rights reserved.