Two new benzylated dihydroflavonols, gericudranins D [1] and E [2], were isolated from the stem bark of Cudrania tricuspidata, and were identified as 5,7,4'-trihydroxy-6,8-di-p-hydroxybenzyldihydroflavonol and 5,7,4'-trihydroxy-6-p-hydroxybenzyldihydroflavonol, respectively. These compounds were shown to be significantly cytotoxic to human tumor cell lines in culture such as CRL 1579 (skin), LOX-IMVI (skin), MOLT-4F (leukemia), KM12 (colon), and UO-31 (renal). Cudrania tricuspidata Bureau (Moraceae) is a deciduous tree that grows in Korea, China, and Japan. This plant has been used as a traditional medicine for neuritis and inflammation in the Orient (1). It has also served as a folk remedy for gastritis and liver damage in Korea. Xanthones (2-6), flavonoids (7-11), and benzenoids (12) have been isolated from the stem and root bark of the plant. In the course of our search for antitumor agents from traditional medicines, three new cytotoxic benzylated dihydroflavonol derivatives were isolated from C. tricuspidata, namely, gericudranins A, B, and C (13). Further purification of an 80% MeOH extract of this plant afforded two more cytotoxic benzylated dihydroflavonols, gericudranins D and E. In this report, their isolation and structure elucidation are reported, along with their cytotoxic evaluation against some human tumor cell lines.