Plasmid-Encoded Carbapenem-Hydrolyzing β-Lactamase OXA-48 in an Imipenem-Susceptible Klebsiella pneumoniae Strain from Belgium

Antimicrobial Agents and Chemotherapy
2008.0

Abstract

Emergence and dissemination of Enterobacteriaceae isolates harboring carbapenemases represent a significant threat to the management of nosocomial infections. The Ambler class D OXA-48 β-lactamase had been found only in enterobacterial species from Turkey. Here we describe a Klebsiella pneumoniae isolate (UCL-1) from a 37-year-old lymphoma patient in Belgium with reduced susceptibility to carbapenems. The isolate was resistant to amoxicillin/amoxicillin-clavulanate, susceptible to meropenem, intermediate to ertapenem, and resistant to fluoroquinolones, cotrimoxazole, kanamycin, and tetracycline. Etest showed imipenem/meropenem susceptibility and ertapenem intermediacy, and a crude β-lactamase extract exhibited significant carbapenem-hydrolyzing activity. PCR and sequencing identified the carbapenemase blaOXA-48 gene, which was surrounded by two IS1999 elements similar to the prototype K. pneumoniae 11978 strain from Turkey. Plasmid analysis detected a 70-kb self-conjugative plasmid (similar to pA-1 in the Turkish strain) that conferred reduced carbapenem susceptibility to Escherichia coli transconjugants. This is the first evidence of OXA-48-type carbapenemase identification outside Turkey in a patient with no apparent relation to the country. Automated systems (Phoenix, Vitek) may miss reduced carbapenem susceptibility, and low-level resistance may occur with additional mutations or plasmid transfer to bacteria with pre-existing reduced carbapenem susceptibility. Ertapenem seems more affected by these enzymes and is a better indicator for their detection.

Knowledge Graph

Similar Paper

Plasmid-Encoded Carbapenem-Hydrolyzing β-Lactamase OXA-48 in an Imipenem-Susceptible Klebsiella pneumoniae Strain from Belgium
Antimicrobial Agents and Chemotherapy 2008.0
Spread of OXA-48-Encoding Plasmid in Turkey and Beyond
Antimicrobial Agents and Chemotherapy 2010.0
Spread of OXA-48-Positive Carbapenem-Resistant Klebsiella pneumoniae Isolates in Istanbul, Turkey
Antimicrobial Agents and Chemotherapy 2008.0
Plasmid-Mediated Carbapenem-Hydrolyzing β-Lactamase KPC-2 in Klebsiella pneumoniae Isolate from Greece
Antimicrobial Agents and Chemotherapy 2008.0
A Plasmid-Borne bla <sub>OXA-58</sub> Gene Confers Imipenem Resistance to Acinetobacter baumannii Isolates from a Lebanese Hospital
Antimicrobial Agents and Chemotherapy 2008.0
Carbapenem-Resistant Acinetobacter baumannii Isolates from Tunisia Producing the OXA-58-Like Carbapenem-Hydrolyzing Oxacillinase OXA-97
Antimicrobial Agents and Chemotherapy 2008.0
Phenotypic and Genotypic Characterization of Enterobacteriaceae with Decreased Susceptibility to Carbapenems: Results from Large Hospital-Based Surveillance Studies in China
Antimicrobial Agents and Chemotherapy 2010.0
Emergence of Serratia marcescens , Klebsiella pneumoniae , and Escherichia coli Isolates Possessing the Plasmid-Mediated Carbapenem-Hydrolyzing β-Lactamase KPC-2 in Intensive Care Units of a Chinese Hospital
Antimicrobial Agents and Chemotherapy 2008.0
First Identification of Pseudomonas aeruginosa Isolates Producing a KPC-Type Carbapenem-Hydrolyzing β-Lactamase
Antimicrobial Agents and Chemotherapy 2007.0
An Ertapenem-Resistant Extended-Spectrum-β-Lactamase-Producing Klebsiella pneumoniae Clone Carries a Novel OmpK36 Porin Variant
Antimicrobial Agents and Chemotherapy 2010.0