The aim of this work was to investigate the antibacterial and antifungal activities of novel D-amino acid-Schiff bases including fluorine atom and their Cr(III) and Ni(II) complexes. All these substances have been examined for antibacterial activity against pathogenic strains Listeria monocytogenes 4b ATCC19115, Staphylococcus aureus ATCC25923, Escherichia coli ATCC1280, Salmonella typhi H NCTC 901.8394, Brucella abortus (A.99, UK-1995) RSKK03026, Staphylococcus epidermis sp., Micrococus luteus ATCC9341, and Shigella dysenteria typ 10 NCTC 9351, and antifungal activity against Candida albicans Y-1200-NIH, Tokyo. The antimicrobial test results of these amino acid-Schiff base complexes exhibited better activity than some known antibiotics. In particular, diamagnetic Ni(II) complexes were more potent bactericides than all of the substances synthesized.