The carbapenems are regarded as the preferential therapeutic option for treatment of serious health care-associated infections with multidrug-resistant gram-negative bacteria. Although carbapenem resistance is rarely described for the Enterobacteriaceae (6), this phenotype of resistance has been increasingly reported worldwide, especially due to the emergence and spread of Klebsiella pneumoniae carbapenemase (KPC) (1, 5, 8). In this report, we describe the first detection of KPC-2-producing K. pneumoniae strains in Brazil. These strains also coproduced an extended-spectrum --lactamase, CTX-M-2.