Effect of various ligands which include Imidacloprid related compounds, on the binding of [3H]phencyclidine (PCP) known as a probe for the allosteric site located in the receptors ion channel and [3H]a-bungarotoxin (a-BGT) known as a probe for the acetylcholine (ACh) recognition site of the nicotinic acetylcholine receptor with its ion channel from the Torpedo electric organ, was examined. Nicotine and anabasine showed a strong agonistic action like carbachol and cytisine, whereas imidacloprid, 6-Cl-PMNI, acetamiprid, NMTHT, nitenpyram and MTENI were weakly agonistic. NMTHT, nitenpyram and MTENI were also noncompetitive blockers like coniine, DMPP, nereistoxin and d-tubocurarine which interacted with both the ACh recognition site and the allosteric site. PCP, TCP, ketamine, chlorpromazine and mecamylamine were noncompetitive blockers; also lobeline and trimethaphan were found to be noncompetitive blockers as against text books.