Flubendiamide is the first benzenedicarboxamide compound developed as an insecticide with potent activity against lepidopterous pests. It has already been clarified that insecticidal activity of the compound is mediated through modulation of the ryanodine-sensitive Ca2 release channel, (ryanodine receptors; RyRs). The present study showed close correlation between insecticidal activities and stimulating effects on the Ca2 pump, a functional co-operative element with RyR activity, and implied the involvement of Ca2 pump stimulation in insecticidal activity. This result suggested that Ca2 pump activity could be a convenient indicator for determining RyR activity.