In this study, we have synthesized 5-(nitro/chloro)-2-styryl-benzimidazoles and evaluated for in vitro anti-microbial activity with different strain like anti-bacterial activity against Staphylococcus aureus, Escherichia coli, and anti-fungal activity against Candida albicans were carried out. Compounds 2, 4, 7, 8 showed higher activity against S. aureus, compounds 2, 8, 9, 10 showed higher activity against E. coli and Compounds 2, 7, 9, 8 showed higher activity against C. albicans.