The microwave-induced three-component onepot synthesis of 2-amino-3-carbethoxy-4-phenylpyrano[3,2-c] quinolin-5(6H)-ones (4a–l) from 4-hydroxyquinolin-2(1H) ones, aromatic aldehydes and ethyl cyanoacetate was described. The detailed synthesis, structure analysis, and antimicrobial screening for the title compounds were also reported. Pharmacological screen studies like anti-inflammatory and antibacterial activities of newly synthesized quinoline derivatives were evaluated against carrageenaninduced rat paw edema model and Gram-positive and Gramnegative bacteria, respectively.