Synthesis and evaluation of small molecules bearing a benzyloxy substituent as novel and potent monoamine oxidase inhibitors

MedChemComm
2017.0

Abstract

A new series of small molecules bearing a benzyloxy substituent have been designed, synthesized and evaluated for hMAO inhibitory activity <i>in vitro</i>. Most of the compounds were potent and selective MAO-B inhibitors, and were weak inhibitors of MAO-A. In particular, compounds <b>9e</b> (IC<sub>50</sub> = 0.35 μM) and <b>10e</b> (IC<sub>50</sub> = 0.19 μM) were the most potent MAO-B inhibitors, and exhibited the highest selectivity for MAO-B (<b>9e</b>, SI > 285.7-fold and <b>10e</b>, SI = 146.8-fold). In addition, the structure-activity relationships for MAO-B inhibition indicated that electron-withdrawing groups in the open small molecules were more suitable for MAO-B inhibition, and substitutions at the benzyloxy of the open small molecules, particularly with the halogen substituted benzyloxy, were more favorable for MAO-B inhibition. Molecular docking studies have been done to explain the potent MAO-B inhibition of the open small molecules. Furthermore, the representative compounds <b>9e</b> and <b>10e</b> showed low neurotoxicity in SH-SY5Y cells <i>in vitro</i>. So the small molecules bearing the benzyloxy substituent could be used to develop promising drug candidates for the therapy of neurodegenerative diseases.

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