Seven highly oxidized steroids, taccachatrones A-G (<b>1</b>-<b>7</b>), together with four known taccalonolides (<b>8</b>-<b>11</b>), were characterized from the rhizomes of <i>Tacca chantrieri</i>. The structures of <b>1</b>-<b>7</b> were established on the basis of spectroscopic data analysis, while the absolute configurations were determined by single-crystal X-ray diffraction. Compounds <b>1</b>-<b>4</b> may be derived from taccalonolide derivatives by the degradation of three carbon atoms. Compounds <b>7</b>, <b>8</b>, <b>10</b>, and <b>11</b> exhibited cytotoxicity to human cancer cell lines, indicating that the presence of a lactone moiety, as well as a double bond between C-22 and C-23, might play key roles in mediating their cytotoxicity.