Two new (<b>1</b> and <b>2</b>) along with six known (<b>3</b>-<b>8</b>) dixiamycins were isolated from the culture broth of a cold-seep-derived actinomycete, <i>Streptomyces olivaceus</i> OUCLQ19-3. Structures of the isolated compounds were elucidated based on extensive MS and NMR spectroscopic analyses together with ECD calculations. In the antibacterial test, compounds <b>1</b>-<b>8</b> exhibited notable growth inhibitions against a panel of multi-drug-resistant (MDR) strains with MIC values of 0.78-6.25 μg/mL, among which <b>1</b>, <b>2</b>, and <b>5</b>-<b>7</b> were more potent than the positive control tetracycline.