Sparticols A (<b>1</b>) and B (<b>2</b>), two catechol-bearing naphthalenedioxy derivatives, were isolated from the submerged culture of the Spanish broom inhabiting Dothideomycetes fungus, <i>Sparticola junci.</i> The structures of <b>1</b> and <b>2</b> were established by NMR spectroscopic analysis and high-resolution mass spectrometry. The 8<i>S</i> absolute configuration of their β-hydroxy functionalities was determined by ECD-TDDFT. Both compounds exhibited inhibitory activity against <i>Staphylococcus aureus</i> with an MIC value of 66.6 μg/mL. Polyketides <b>1</b> and/or <b>2</b> may be associated with pathways cascading to <i>seco</i>-spirodioxynapthalene derivatives.