Three novel furo-naphthoquinones, enceleamycins A-C (<b>1</b>-<b>3</b>), and a new <i>N</i>-hydroxypyrazinone acid (<b>4</b>) were identified from the strain <i>Amycolatopsis</i> sp. MCC 0218, isolated from a soil sample collected from the Western Ghats of India. Their chemical structure and absolute and relative configurations were established by 1D and 2D NMR spectroscopy, single-crystal X-ray crystallography, and high-resolution mass spectrometry. Compounds <b>1</b> and <b>3</b> were active against methicillin-susceptible and -resistant <i>Staphylococcus aureus</i> with MIC values of 2-16 μg/mL.