Seven new 18-residue peptaibols, trichorzins A-G (<b>1</b>-<b>7</b>), were isolated from the sponge-derived fungus <i>Trichoderma</i> sp. GXIMD 01001. Their structures and configurations were characterized by a comprehensive interpretation of the NMR spectroscopic data, MS/MS fragmentation, Marfey's method, and ECD analysis. The general sequences of trichorzins A-G are as follows: Ac-Aib<sup>1</sup>-Ala/Ser<sup>2</sup>-Ala<sup>3</sup>-Aib/Iva<sup>4</sup>-Iva<sup>5</sup>-Gln<sup>6</sup>-Aib/Iva<sup>7</sup>-Val/<i>allo</i>-Ile<sup>8</sup>-Aib<sup>9</sup>-Gly<sup>10</sup>-Leu<sup>11</sup>-Aib<sup>12</sup>-Pro<sup>13</sup>-Leu<sup>14</sup>-Aib<sup>15</sup>-Aib<sup>16</sup>-Gln<sup>17</sup>-Trpol/Pheol<sup>18</sup>. The obtained compounds were assessed for their potential antiproliferative and antimicrobial activities. All obtained compounds did not show potent antibacterial activity but exhibited significant cytotoxicity, with the lowest IC<sub>50</sub> values at 0.46-4.7 μM against four human carcinoma cell lines.