Multiplex assay results for EUbOPEN Chemogenomics Library: The multiplex high-content screen is used to investigate the cytotoxicity of compounds in different cell lines such as HEK293T, U2OS, and MRC-9 cells. This assay, detects compound properties like precipitation or intrinsic fluorescence, cell viability as well as the effect on tubulin, mitochondria or membrane integrity by staining living cells with different fluorescent dyes. Different compound libraries containing of probes or chemogenomic compounds are tested over a kinetic measurement at 0h, 12h, 24h and 48h. Cell sub-populations are gated by a machine-learning-guided algorithm using the CellPathfinder Software (Yokogawa).