Corydalis stricta Steph. is a plant of the genus Corydalis Vent. (family Fumariaceae DC.) growing in Central Asia and Western Siberia. Previous studies reported alkaloids (protopine, (+)-β-hydrastine, sanguinarine, etc.) in its aerial parts and roots, but its seeds remained unstudied. Irgashev et al. had isolated 23 alkaloids from roots, stems, leaves of Pamir-sourced (USSR) plants and aerial parts of Mongolian-sourced plants. This investigation represents the first study on the seeds of C. stricta from the same Soviet Pamir region. The alkaloid content of the seeds was found to be 1.04%. Isolated alkaloids included phtalidisoquinoline alkaloids (bicuculline as the major component, (+)-β-hydrastine, adlumidine), protopine alkaloid (protopine), protoberberine alkaloids (scoulerine, stylopine), and quaternary alkaloids (cis-N-methylstylopinium hydroxide iodide, pycnarrhine (1,2-dehydrocorypallinium hydroxide) iodide). All these alkaloids had been previously identified in roots or aerial parts of C. stricta studied by Irgashev et al. However, other alkaloids reported in roots/aerial parts of Pamir-sourced plants (coreximine, isoboldine, juziphine, corypalline, sanguinarine, wilsonirine, pancorine, pancoridine, N-methylcorypallinium hydroxide) and aerial parts of Mongolian-sourced plants (cheilanthiofoline, isocorypalmine, isoboldine, reticuline, N-methylcoclaurine, sanguinarine, dihydrosanguinarine, adlumine) were not detected in the seeds.