Pht cell cultures, 176G, 299Y, 340T and 95lG, from C. roseus gave the following alkaloids: yohimbine (5), isositsirikine (6), horhammericine (7), horhammerinine (8), vindolinine (9), 19-epivindolinine (lo), ajmalicine (3), lochnericine (14). vallesiachotamine (a), strictosidine (12), strictosidine lactam (111, lochrierinine (15) and N,N-dimethyl-tryptamine (13). In recent publications (1-6) we have reported the production of a large number of indole alkaloids both in callus and suspension cultures derived from Catharaitllrus roseus. Among these cell cultures there have been cell lines that produce catharanthine (1, Iboga) and a variety of -4spidosperma and Corynanthk alkaloids. In a recent publication Stockigt et. al. ('7) have also reported the production of cultures that synthesize the more biosynthetically complex indole alkaloids. It was interesting to note that Stockigt found, with a fen- minor differences, the same alkaloids that we had earlier isolated and fully characterized.In our continued investigations for cell lines that produce catharanthine (1) and/or vindoline (2), n-e have been examining the differences found in alkaloid production from cell cultures that have been derived, by anther explants, from different C. roseus plants. By following this approach we hoped to propagate a cell line that could synthesize a single or, perhaps, tno major components. A cell line that could produce predominantly a single chemo-therapeutic component would have considerable significance for the pharmaceutical industry. Here we describe some of the results so far obtained.