<jats:title>Abstract</jats:title><jats:p>The 953 cell line, one of the cell lines from <jats:italic>Catharanthus roseus</jats:italic>, has been investigated in terms of its ability to produce indole alkaloids. Various parameters (mitotic index, pH, cell dry weight, growth period) were evaluated in the fermentation process and their relative importance for alkaloid production was determined. The alkaloids isolated and identified are ajmalicine, yohimbine, isositsirikine, vallesiachotamine, strictosidine lactam, lochnericine, hörhammericine, hörhammerinine, vindolinine, 19‐epivindolinine, 19‐acetoxy‐11‐methoxytabersonine, 19‐hydroxy‐11‐methoxytabersonine and dimethyltryptamine.