<jats:title>ABSTRACT</jats:title> <jats:p> The filamentous fungus <jats:italic>Acremonium chrysogenum</jats:italic> is the industrial producer of the β-lactam antibiotic cephalosporin C. Here, we present the genome sequence of strain ATCC 11550, which contains genes for 8,901 proteins, 127 tRNAs, and 22 rRNAs. Genome annotation led to the prediction of 42 gene clusters for secondary metabolites.