Literature

Abstract

In an attempt to construct a strain that produces doramectin, the loading module of Ave polyketide synthase (PKS) from Streptomyces avermitilis M1 was replaced with a cyclohexanecarboxylic (CHC) unique loading module from phoslactomycin PKS. Additionally, the CHC-CoA biosynthetic gene cassette was introduced into the engineered strain, which provided the precursor for directed biosynthesis of doramectin. The doramectin production ability of the final mutant S. avermitilis TG2002 was increased about six times and the ratio of Dor to Ave was enhanced 300 times more than the original strain.

DOI： 10.1016/j.bmcl.2011.04.008

Production of doramectin by rational engineering of the avermectin biosynthetic pathway

Bioorganic & Medicinal Chemistry Letters 2011.0

Engineering the aveC gene to enhance the ratio of doramectin to its CHC‐B2 analogue produced in Streptomyces avermitilis

Biotechnology and Bioengineering 2003.0

Semi-synthetic DNA shuffling of aveC leads to improved industrial scale production of doramectin by Streptomyces avermitilis

Metabolic Engineering 2005.0

Engineered biosynthesis of milbemycins in the avermectin high-producing strain Streptomyces avermitilis

Microbial Cell Factories 2017.0

Construction of ivermectin producer by domain swaps of avermectin polyketide synthase in Streptomyces avermitilis

Applied Microbiology and Biotechnology 2006.0

Direct production of ivermectin-like drugs after domain exchange in the avermectin polyketide synthase of Streptomyces avermitilis ATCC31272