In vitro cultures were initiated from different parrs ofthe plant Ranzwlhserpentinu. Leaf and stem explants gave actively growing callus tissues on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) (2 mg/liter)+ benzyladenine (BA) (1 mg/liter). Multiple shoots developed from axillary meristem explants on MS medium supplemented with BA in combination with naphthaleneacetic acid (NM) or indoleacetic acid (IAA). Sustained growth of the shoot cultures was achieved on MS liquid medium containing (BA+NM). The shoot cultures produced significantly high levels of alkaloids. One of the isolated alkaloids was characterized as 3-epi-a-yohimbine.