Roots of Delphinium cuneatum collected on Belebeev plateau (Bashkortostan, Aslykul/Kandrykul area, flowering phase) were studied. We have previously reported the isolation from this plant of the new alkaloid 16-demethoxymethyllycaconitine (1). A second base (2) that has not been previously described in the literature was isolated during further work on alkaloid separation. The mass spectrum of 2 gave a peak for the molecular ion at 684 [M]+. According to IR spectra, 2 contains an ester (1720 cm-1) and amides (1540 and 1590 cm-1). Alkaline hydrolysis of 2 produced aminoalcohol 3, the spectral properties (PMR and 13C NMR) of which were identical to those described earlier for 16-demethoxylycoctonine, the product of alkaline hydrolysis of 1. Thus, 1 and 2 differed by the substituent on C18. The PMR spectrum of 2 exhibited two doublets at 1.29 and 1.35 ppm (J = 6.9 Hz) and singlets for two ester methoxyls at 3.69 and 3.72 ppm. The 13C NMR spectrum using JMODCH for 2 gave signals belonging to the C-18 substituent, some of which were doubled such as 114.6/114.7, 141.6/141.8, 51.8/52.0, and 17.9/17.1 ppm. The spectral data suggested that 2 was a pair of isomers 2a and 2b. The alkaloid delavaine (4) has been reported, which is also a mixture of two isomers that differ from 2 by the presence of a C16 methoxy. Thus, 2 is 16-demethoxydelavaine. A mixture of two new norditerpene alkaloids consisting of two regioisomers was isolated from the total alkaloids of Delphinium cuneatum roots, and their structures were proposed as 16-demethoxydelavaine (2a and b) on the basis of PMR, 13C NMR, IR, and mass spectra.