Acuminatopyrone, a recently described metabolite of Fusarium aurninatum, was shown to be produced by several strains of Fusarium chlamydosponrm and F. tricinctum isolated from various substrates in different countries. The structure of acuminatopyrone has been revised on the basis of NOEDIF and HETCOR nmr experiments, and shown to be 4-methoxy-7,8 dimethyl-2H-pyrano[4,3-b]pyridin-2-one. This structure is biosynthetically consistent with that of chlamydosporol and related compounds that are produced by the same fungal species.