Acrodontiolamide, a new fungal metabolite has been isolated from the cell-free culture medium of Acrodonfium salmoneum. Its purification was achieved by centrifugal TLC on silica gel and the structural analysis was based on spectroscopic data relative to both the natural product and its diacetyl derivative. This secondary metabolite, identified with 3-(pnitrophenyl)-3-hydroxy-4-dichloromethoxy-isobutanamide exhibited antifungal properties.