The alkaloids of tall fescue hay (Festuca arscndinacea Schreb.) have been esamined as apart of our work on the etiology of a cattle disease known as fescue foot. Perloline has been isolated from tall fescuG, and the presence of ergot-like alkaloids has been indicated by color tesW. However, no references have been found on the systematic separation of fescue alkaloids. This communication describes the separation of nine alkaloids by paper chromatography, and a method for isolating certain individual alkaloids.