The individual alkaloids present in the total alkaloids extracted from the sclerotia and saprophytic cultures of some ergot fungi have been qualitatively determined; the water-insoluble alkaloids by preparing chromatograms from the acid and alkali hydrolysates of their fraction, while the water-soluble alkaloids directly by the ascending paper partition chromatography, using Toyo No. 131 filter paper treated with 0.1M citric acid-0.2M disodium hydrogen phosphate buffer (MCILVAINE) of pH 7.2∼7.6, and developing with ether saturated with water at 7∼8°. The results are summarized in Tables 4 and 5. From the data, it has been concluded that any ergot fungus could produce, both in nature and in saprophytic culture, all kinds of ergot alkaloids including four unknown alkaloids (the water-insoluble alkaloids “P1” and “P2” and the water-soluble alkaloids “X” and “Y”) discovered in the course of the present work. Addendum:-After we had reported the present work at the Annual Meeting of Agr. Chem. Soc. Japan held at University of Tokyo on April 5, 1952, some reports concerning the method for the separation of ergot alkaloids by paper partition chromatography were given by many investigators, such as J. E. CARLESS [J. Pharm. Pharmacol., 5, 883 (1953)], M. PÖHIM and L. FUCHS [Naturwiss., 41, 63 (1954)], K. MACEK. A. CERNY and M. SEMONSKY [Pharmazie, 9, 388 (1954)], J. TUZSON and G. VASTAGH [Pharm. acta Helv., 29, 118, 357 (1954)] and A. STOLL and A. RUEGGER [Hely. Chim. Acta, 37, 1725 (1954)]. Moreover, M. PCHM reported [Monatsh., 85, 1010 (1954)] that he has discovered two unknown alkaloids, ergohexine and ergoheptine by the chromatographic method of POHM and FUCHS, in the sclerotia of Elymus mollis TRIN. We are convinced that these two alkaloids are nothing but the alkaloids “P1” and “P2” mentioned above. © 1955, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.