The new antiinsectan metabolite 2 (10-oxo-11,33-dihydropenitrem B) has been isolated from the sclerotia of Aspergillus sulphureus. Compound 2 is related to the penitrems, a known group of tremorgenic fungal metabolites. The structure of 2 was assigned primarily by NMR studies (e.g., COSY, HMQC, HMBC, and NOESY) and by comparison to data published for the penitrems. A known aflavinine analog (3; 10,23-dihydro-24,25-dehydroaflavinine) was also isolated from A. sulphureus sclerotia. This is the first report of any aflavinine analog from a member of the Aspergillus ochraceus taxonomic group.