<jats:title>Abstract</jats:title> <jats:p>Five new alkaloids have been isolated from the plant Daphniphyllum gracile Gage, and their structures also been elucidated on the basis of their spectral and chemical evidences. Particularly, daphnigraciline methiodide (VI) was directly converted into yuzurine methiodide (VII). Furthermore, application of 13C NMR spectra plays an important role in the structural elucidation.