A new spermidine triamide derivative has been isolated from peanut flowers and identified as N1 acetyl-N5 ,N10-di-p-(EE)-coumaroylspermidine on the basis of detailed analysis of NMR, MS, and UV data. Two other spermidine conjugates, N1 ,N5 ,N10-tri-p-(EEE)-coumaroylspermidine and di-p-(EE) coumaroylspermidine, as well as four flavonoid conjugates (quercetin-3-glucoside, quercetin-3 glucuronide, isorhamnetin-3-glucoside, and isorhamnetin-3-glucuronide) that have been previously reported in organs of other plants, have been found in this study in peanut (Arachis hypogaea L.), a representative of the Leguminosae family, for the first time. The dynamics of photoisomerization in the spermidine conjugates have been investigated.