Lactoquinomycin, a new antibiotic, C24H27NO8, mp 151 approximately 159 degrees C (dec), FAB-MS: m/z 458 (MH+), is a basic substance, showing UV lambda MeOHmax (epsilon) 215 (37,600), 254 (10,700) and 432 nm (4,760), and IR nu CHCl3max 1790 (gamma-lactone), 1665 and 1650 (quinone) cm-1. The structure of lactoquinomycin has been elucidated by 1H NMR and ORD in comparison with those of kalafungin.