Bioassay-guided fractionation of the MeOH extracts of Euphorbia hirta Linn (Euphorbiaceae) aerial parts led to the isolation of flavonol glycosides afzelin (1), quercitrin (2), and myricitrin (3), whose structures were established by MS and NMR analysis. Compounds 1–3 showed proliferation inhibition of Plasmodium falciparum, with IC50 values of 1.1, 4.1, 5.4 mg=mL, repectively. On the other hand, they exhibited little cytotoxic property against human epidermoid carcinoma KB 3-1 cells.