The purity of five laboratory and five industrial batches of the pharmacopoeial preparation of anabasine hydrochloride and five laboratory batches of standard anabasine hydrochloride was checked by the above-described method. No N-nitrosoanabasine was detected in any of the batches, which shows the high purity of the pharmacopoeial preparation and of the standard anabasine hydrochloride. From the combined alkaloids extracted by ethers from the roots and rhizomes of Veratrum nigrum L., five alkaloids were isolated by chromatography on a column of cellulose. The compounds were identified by the methods used for the alkaloids of Veratrina Zobelium Bernh., and the results showed that they were dideacetylprotoveratrine A (I), veramarine (II), germidine (III), protoveratrine A (IV), and verazine (V). This is the first time that these alkaloids have been found in Veratrum nigrum.