In our efforts to develop efficient and faster methods for the isolation of pure compounds from crude mixtures of alkaloids, we report here the separation of some closely related diterpenoid alkaloids on the Chromatotron, a centrifugally accelerated, radial, thin-layer chromatography (TLC) instrument. The use of silica rotors for the separation of xanthones, triterpenes, saponins and retinoids has been documented in earlier literature, but the separation of alkaloid mixtures using silica or alumina rotors has not been reported. The techniques of separation of complex diterpenoid alkaloids of Aconitum, Delphinium (Ranunculaceae) and Garrya (Garryaceae) species has been recently discussed. Most of the methods used for the isolation of alkaloids in a pure state are laborious and time consuming. In connection with our work on the isolation of alkaloids from Delphinium and Aconitum species we have evaluated the Chromatotron for its suitability for achieving rapid preparative separations.