The use of a Chromatotron instrument in the separation of complex mixtures of natural products has been reported1,2, mostly using silica gel layers. Our involvement in the use of chromatography for the isolation of pure natural products from plants prompted a study of other materials and applications for centrifugal thin-layer chromatography. We obtained good results in the separation of polar substances such as triterpene and flavonoid glycosides using a "starch-bound" layer2-4. In this paper we report the application of a basic layer for the one-step isolation of up to five closely related alkaloids from the same vegetable source. To the best of our knowledge, only two papers on the centrifugal thin-layer chromatography (CTLC) of alkaloids using a different type of alumina5,6 have been published.